Following co-transcriptional endonculeolytic cleavage of the nascent 35S precursor by Rnt1, the remaining 5’ RNA fragment still associated with Pol I recruits 5’-3’ exonuclease Rat1, which then catches and “torpedoes” the polymerase at a downstream pause site resulting in its release from the DNA template. As this mechanism shows striking similarities to the “torpedo” model for Pol II termination on mRNAs, it confirms that two different polymerases utilize parallel mechanisms of transcription termination. Currently we carry on this line, investigating the involvement of the Nrd1/Nab3 complex in PolI termination.
(in collaboration with David Tollevery, The Wellcome Trust Centre for Cell Biology, University of Edinburgh, Scotland)
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(in collaboration with David Tollevery, The Wellcome Trust Centre for Cell Biology, University of Edinburgh, Scotland)
Back to Yeast Projects